| Rnk | Bib |  | Name | Nation | Bike | End of Bike |
| 1 | 20713 | | Cindi Bannink | USA | 33:29.2 (0) | 46:42.5 |
| 2 | 20695 | | Claire Vallance | GBR | 33:48.7 (0) | 47:07.0 (+24.4) |
| 3 | 20714 | | Diane Hankee | USA | 34:06.7 (0) | 49:58.6 (+3:16.0) |
| 4 | 20694 | | Sally Baldwin | GBR | 34:27.0 (0) | 50:32.1 (+3:49.5) |
| 5 | 20722 | | Nicole Annson | AUS | 35:39.5 (0) | 51:20.0 (+4:37.4) |
| 6 | 20710 | | Renee Ker | NZL | 36:45.1 (0) | 51:54.2 (+5:11.6) |
| 7 | 20665 | | Heather Dart | CAN | 37:17.3 (0) | 52:37.1 (+5:54.5) |
| 8 | 20724 | | Tania Gover | AUS | 37:23.3 (0) | 52:41.1 (+5:58.5) |
| 9 | 20688 | | Amanda Yates | CAN | 37:37.3 (0) | 53:01.5 (+6:19.0) |
| 10 | 20677 | | Lianne Mclellan | CAN | 36:21.0 (0) | 53:19.4 (+6:36.8) |
| 11 | 20690 | | Claire Lloyd | GBR | 38:09.3 (0) | 53:52.6 (+7:10.0) |
| 12 | 20670 | | Joanna Jackson | CAN | 38:26.3 (0) | 54:05.8 (+7:23.3) |
| 13 | 20681 | | Irene Rey | CAN | 38:20.0 (0) | 54:22.5 (+7:39.9) |
| 14 | 20692 | | Louise Rawlinson | GBR | 38:33.5 (0) | 54:24.8 (+7:42.2) |
| 15 | 20693 | | Sally Boon | GBR | 38:48.5 (0) | 54:24.8 (+7:42.2) |
| 16 | 20718 | | Nicole Brown | USA | 36:45.8 (0) | 54:31.2 (+7:48.7) |
| 17 | 20680 | | Suzie Poirier | CAN | 36:39.0 (0) | 55:22.4 (+8:39.9) |
| 18 | 20678 | | Linda Mittelstadt | CAN | 38:28.8 (0) | 55:24.0 (+8:41.4) |
| 19 | 20716 | | Rocio Ruiz | USA | 38:28.4 (0) | 55:55.7 (+9:13.2) |
| 20 | 20715 | | Shannon Grady | USA | 40:15.1 (0) | 56:01.8 (+9:19.3) |
| 21 | 20709 | | Michelle Lyons | NZL | 40:36.8 (0) | 56:10.9 (+9:28.3) |
| 22 | 20691 | | Kathy Wellam | GBR | 40:46.0 (0) | 56:22.7 (+9:40.1) |
| 23 | 20674 | | Erin Linton | CAN | 40:54.5 (0) | 57:01.3 (+10:18.7) |
| 24 | 20672 | | Dayna Kucy | CAN | 39:04.5 (0) | 57:28.6 (+10:46.0) |
| 25 | 20683 | | Tina Seifert | CAN | 37:43.6 (0) | 57:37.6 (+10:55.0) |
| 26 | 20696 | | Lilia Alejandra Gomez Dominguez | MEX | 40:35.6 (0) | 57:54.5 (+11:11.9) |
| 27 | 20712 | | Summer Rehkemper | USA | 39:12.0 (0) | 58:05.7 (+11:23.1) |
| 28 | 20675 | | Sheri Bentley | CAN | 38:42.9 (0) | 58:12.5 (+11:29.9) |
| 29 | 20684 | | Jay Smith | CAN | 41:43.0 (0) | 58:17.5 (+11:34.9) |
| 30 | 20703 | | Hazel Mejia Herrada | MEX | 41:02.1 (0) | 58:25.8 (+11:43.2) |
| 31 | 20721 | | Hannah Ryan | AUS | 41:00.4 (0) | 58:33.3 (+11:50.7) |
| 32 | 20726 | | Emily Loterio Barcelos Maciel | BRA | 40:08.9 (0) | 58:36.0 (+11:53.4) |
| 33 | 20664 | | Karen Cambaliza | CAN | 40:50.4 (0) | 58:36.7 (+11:54.1) |
| 34 | 20708 | | Melanie Anders | NZL | 40:39.8 (0) | 58:38.1 (+11:55.5) |
| 35 | 20671 | | Jodi Kresowaty-Harris | CAN | 41:31.1 (0) | 58:53.4 (+12:10.8) |
| 36 | 20666 | | Deidre Derworiz | CAN | 40:50.5 (0) | 58:54.9 (+12:12.3) |
| 37 | 20679 | | Natalie Morse | CAN | 39:45.1 (0) | 59:28.3 (+12:45.7) |
| 38 | 20719 | | Melissa Guthrie | USA | 41:43.3 (0) | 59:45.5 (+13:02.9) |
| 39 | 20676 | | Tara Mcaninch Mclaren | CAN | 40:17.1 (0) | 59:49.6 (+13:07.0) |
| 40 | 20682 | | Jennifer Seaman | CAN | 41:20.0 (0) | 59:50.9 (+13:08.3) |
| 41 | 20707 | | Jessica Astridge | NZL | 40:48.0 (0) | 59:55.7 (+13:13.1) |
| 42 | 20717 | | Kristi Donovan-Wheeler | USA | 41:51.5 (0) | 59:57.9 (+13:15.3) |
| 43 | 20667 | | Melanie Ducholke | CAN | 42:34.5 (0) | 1:00:09.9 (+13:27.3) |
| 44 | 20668 | | Carmen Gill | CAN | 40:48.0 (0) | 1:00:32.8 (+13:50.2) |
| 45 | 20685 | | Jill Sorenson | CAN | 43:30.5 (0) | 1:00:47.4 (+14:04.9) |
| 46 | 20697 | | America Serrano Rodriguez | MEX | 41:37.5 (0) | 1:01:07.6 (+14:25.0) |
| 47 | 20689 | | Jennifer Zawaski-Dellezay | CAN | 42:27.5 (0) | 1:01:18.0 (+14:35.4) |
| 48 | 20706 | | Ynna Altair Lopez Vara | MEX | 43:01.3 (0) | 1:01:50.4 (+15:07.8) |
| 49 | 20700 | | Cynthia Gutierrez Etienne | MEX | 41:09.5 (0) | 1:02:27.4 (+15:44.8) |
| 50 | 20687 | | Jenn Wilson | CAN | 43:53.8 (0) | 1:02:51.0 (+16:08.4) |
| 51 | 20673 | | Melanie Lessard | CAN | 44:41.0 (0) | 1:02:56.5 (+16:13.9) |
| 52 | 20704 | | Rosa Delia Gomez Vidal | MEX | 43:45.1 (0) | 1:03:30.4 (+16:47.9) |
| 53 | 20699 | | Bertha Angelica Pulido Martinez | MEX | 46:10.5 (0) | 1:06:06.2 (+19:23.6) |
| 54 | 20686 | | Erin Thibault | CAN | 54:39.8 (0) | 1:18:49.9 (+32:07.3) |
